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biotinylated polyclonal sheep anti human adamts 13  (R&D Systems)


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    R&D Systems biotinylated polyclonal sheep anti human adamts 13
    Biotinylated Polyclonal Sheep Anti Human Adamts 13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+polyclonal+sheep+anti+human+adamts+13/Human+ADAMTS13+Biotinylated+Antibody/10__1017_slash_s0022149x19000798-57-35-42
    Average 90 stars, based on 1 article reviews
    biotinylated polyclonal sheep anti human adamts 13 - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: Haemostatic changes in urogenital schistosomiasis haematobium: a case-control study in Gabonese schoolchildren
    Article Snippet: Used standards were serial dilutions of normal pooled plasma (Sanquin, Amsterdam, The Netherlands) with a known concentration of VWF:ag for VWF:ag and ADAMTS-13:ag, normal pooled serum with known concentrations of TAT and OPG for TAT and OPG ELISAs and VWF-depleted plasma (Affinity Biologicals, Ancaster, Canada) supplemented with recombinant R1341QVWF for active VWF ELISA. .. Following sample incubation, the plates were incubated at room temperature for one hour with the following detection and secondary antibodies diluted in blocking buffer: peroxidase-conjugated rabbit-anti-human VWF (0.325 μg/ ml), peroxidase-conjugated rabbit-anti-human VWF (1.2 μg/ml), biotinylated polyclonal sheep-anti-human ADAMTS-13 (0.25 μg/ ml; R&D Systems, Minneapolis, MN, USA) and streptavidinmono-horseradish peroxidase (HRP) (0.83 μg/ml; DAKO, Carpinteria, CA, USA), biotinylated goat-anti-human OPG/ TNFRSF11B (100 ng/ml; R&D Systems, Minneapolis, MN, USA) and streptavidin-mono-HRP (0.25 μg/ml), and peroxidaseconjugated sheep-anti-human antithrombin (0.5 μg/ml; Stago BNL, Leiden, The Netherlands). .. ELISAs were developed with SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific, Waltham, MA, USA) and luminescence was detected with the Synergy2 ELISA reader (BioTek, Winooski, VT, USA).

    Blocking Assay:

    Article Title: Haemostatic changes in urogenital schistosomiasis haematobium: a case-control study in Gabonese schoolchildren
    Article Snippet: Used standards were serial dilutions of normal pooled plasma (Sanquin, Amsterdam, The Netherlands) with a known concentration of VWF:ag for VWF:ag and ADAMTS-13:ag, normal pooled serum with known concentrations of TAT and OPG for TAT and OPG ELISAs and VWF-depleted plasma (Affinity Biologicals, Ancaster, Canada) supplemented with recombinant R1341QVWF for active VWF ELISA. .. Following sample incubation, the plates were incubated at room temperature for one hour with the following detection and secondary antibodies diluted in blocking buffer: peroxidase-conjugated rabbit-anti-human VWF (0.325 μg/ ml), peroxidase-conjugated rabbit-anti-human VWF (1.2 μg/ml), biotinylated polyclonal sheep-anti-human ADAMTS-13 (0.25 μg/ ml; R&D Systems, Minneapolis, MN, USA) and streptavidinmono-horseradish peroxidase (HRP) (0.83 μg/ml; DAKO, Carpinteria, CA, USA), biotinylated goat-anti-human OPG/ TNFRSF11B (100 ng/ml; R&D Systems, Minneapolis, MN, USA) and streptavidin-mono-HRP (0.25 μg/ml), and peroxidaseconjugated sheep-anti-human antithrombin (0.5 μg/ml; Stago BNL, Leiden, The Netherlands). .. ELISAs were developed with SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific, Waltham, MA, USA) and luminescence was detected with the Synergy2 ELISA reader (BioTek, Winooski, VT, USA).



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    90
    R&D Systems biotinylated polyclonal sheep anti human adamts 13
    Biotinylated Polyclonal Sheep Anti Human Adamts 13, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+polyclonal+sheep+anti+human+adamts+13/Human+ADAMTS13+Biotinylated+Antibody/10__1017_slash_s0022149x19000798-57-35-42
    Average 90 stars, based on 1 article reviews
    biotinylated polyclonal sheep anti human adamts 13 - by Bioz Stars, 2026-09
    90/100 stars
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